Chen and co-workers from the Sun Yat-sen Universits, Guangong, China, Queensland University of Technology, Australia, University of Adelaide Australia, and the University of South Australia, investigated how the application of nanotopography affect macrophags, thereby modulates the osteo-immune response, and consequently also osteogenesis.
The found that tuning the surface chemistry (amine or acrylic acid) and the scale of the nanotopography (16, 38, and 68 nm) significantly modulated the osteoimmune environment, including changes in the expression of inflammatory cytokines, osteoclastic activities, and osteogenic, angiogenic, and fibrogenic factors. The generated osteoimmune environment significantly affected the osteogenic differentiation of bone marrow stromal cells, with carboxyl acid-tailored 68 nm surface nanotopography offering the most promising outcome.
The authors concluded that the study demonstrated that the osteoimmunomodulation could be manipulated via tuning the chemistry and nanotopography, which implied a valuable strategy to apply a “nanoengineered surface” for the development of advanced bone biomaterials with favourable osteoimmunomodulatory properties.
Congratulations to the authors for such an excellent example of high-quality research.
The study is available online only with subscription: http://pubs.acs.org/doi/full/10.1021/acsnano.6b07808
However, the abstract and plenty of supporting information is available free of charge on the ACS Publications website at DOI: 10.1021/acsnano.6b07808, such as detailed experimental procedures; RT-qPCR primers; macrophage morphology under light microscopy, confocal, and SEM; macrophage LC3 fluorescent staining; intracellular autophagy structures observed by TEM; multinucleated cells observed by confocal; morphology of BMSCs under light microscopy; activation of BMP pathway by RT-qPCR; schematic illustration of intracellular response in macrophages (PDF).